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Sis of the Cul5 allele. 2RT indicates that no reverse transcriptase was added. E, Immunoblot comparing Roc2 protein levels in wild-type (w1118) and homozygous Roc2KG embryos. F, Immunoblot comparing Cul5 protein levels in wild-type and homozygous Cul5EY embryos. In each case the embryos were derived from crosses between mutant mothers and fathers. G, Embryo extracts from Cul5 and Roc2 mutants wer
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