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Protein-A beads were washed 36 with 1 ml NP-40 lysis buffer, and then pre-incubated with antibody for 2 h before adding to 1 ml of lysate (1 mg/ml protein). Immunoprecipitations were performed overnight at 4uC.Creation of GST-fusion ProteinsUsing the pCaSpeR-4 Roc constructs as template [25], PCR products were made using primers that added EcoR1 sites on either side, and the product was then clon
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