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A was verified by agarose gel electrophoresis. To verify differential expression of coagulation related ESTs we chose nine EST transcripts from the B. schlosseri rejection EST library for RT-PCR reactions. First strand cDNA was synthesized by DNA synthesis kit (Fermentas, MD, USA). The PCR amplification was performed using designed sets of primers (Metabion, Martinsried, Germany) as depicted in T
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